نوع مقاله: اصیل پژوهشی
نویسندگان
1 دانش آموخته کارشناسی ارشد علوم و صنایع غذایی، دانشکده علوم و مهندسی صنایع غذایی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران.
2 گروه علوم وصنایع غذایی، دانشکده علوم و مهندسی صنایع غذایی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران، ایران
3 مرکز تحقیقات سلامت مواد غذایی و آشامیدنی، دانشگاه علوم پزشکی ارومیه، ارومیه، ایران
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Synthetic preservatives have been used as food additives to extend shelf life of foods, but they are strictly regulated due to toxicological concerns and some health problems. So it is increasingly attractive to find out effective and nontoxic measures (e.g. use of natural antimicrobial agent) to delay microbial spoilage. Nisin is a bacteriocin that it is the only bacteriocin permitted for use in foods. And rosemary is one of the medicinal plants. In this study the effect of antibacterial property of various concentration of rosemary extract alone (3 and 4 mg/ml), and with nisin (7 µg/ml nisin + 1.5 mg/ml RE and 8 µg/ml nisin + 2 mg/ml RE) against Staphylococcus aureus in minced meat were assessed throughout 14 days of storage at 4±1˚C. Leaves and stems of rosemary after drying in the shade were soaking in pure ethanol and alcoholic extract was obtained. The minimum inhibitory concentration (MIC) of RE and nisin were determined against S. aureus using the agar dilution method. Assessment of microbial parameters (total plate count and S. aureus count) was carried out in 0, 1, 3, 7 and 14 days. The data were statistically significant (P<0.05). The results reveals that treatment with both concentrations of RE showed significantly lower microbial indexes in comparison with controls, but treatment with both preservative (nisin & RE) had better results and results were statistically significant (P<0.05). As a consequence, using of RE with nisin was better than of RE alone.
کلیدواژه‌ها [English]
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