نوع مقاله: پژوهشی
نویسندگان
1 دانش آموخته کارشناسی ارشد، گروه میکروبیولوژی، دانشکده علوم پایه، واحد سیرجان، دانشگاه آزاد اسلامی، سیرجان، ایران
2 گروه میکروبیولوژی، دانشکده علوم پایه، واحد ساوه، دانشگاه آزاد اسلامی، ساوه، ایران.
3 مرکز تحقیقات میکروب شناسی پزشکی، دانشگاه علوم پزشکی قزوین، قزوین، ایران.
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Today,food borne diseases are one of the most serious problems and occur as a result of consumption to contaminated food and water. The aim of the study was evaluation of virulence and enterotoxin genes in Salmonella enteritidis strains isolated from food samples by multiplex-PCR. This descriptive, cross-sectional study was performed at 2014 on the 1250 no duplicative and non-repetitive food samples. M-PCR assay was done in order to detection of Stn،sopB،slyA،spvc and Phop/Q genes. Sixty Salmonella enteritidis strains were obtained from poultry meat (35 strains, 58.3%) and eggs (25 strains, 41.6%), respectively. molecular analysis distribution showed all isolates (100%) were absence for spvC gene. The highest and lowest prevalence of the genes were related to Phop/Q and SopB,33.3% and 1.6%, respectively. Evaluation of virulence genes and enterotoxin in the Salmonella enteritidis isolated from the food samples are useful because of presence of the genes and efficacy of M-PCR method in epidemiological investigation and assessment of intraspecies genes transfer in food samples.
کلیدواژه‌ها [English]
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