نوع مقاله : اصیل پژوهشی
نویسندگان
1 گروه میکروبیولوژی، دانشکده علوم زیستی، دانشگاه آزاد اسلامی، واحد تهران شمال، تهران، ایران
2 گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد ساوه، ساوه، ایران
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Klebsiella pneumoniae is a Gram-negative, non-motile, encapsulated, lactose-fermenting, facultatively anaerobic, rod-shaped bacterium.It has traditionally been considered an opportunistic pathogen and is a common cause of nosocomial infections. The pks gene cluster encodes enzymes responsible for the synthesis of colibactin. Colibactin is a genotoxin that has been shown to induce DNA damage and contribute to increased virulence. Colibactin is also strongly suspected of being involved in the development of colorectal cancer. The present study investigated the prevalence of pks, clbN, and clbB genes in Klebsiella pneumoniae isolates. In this study, 220 samples were obtained from raw milk. Then, all samples were cultured in the violet red bile agar and then cultured in blood agar, MacConkey agar, and chocolate agar for bacterial isolation. Biochemical and microbiological tests were performed for confirmation of the bacteria. DNA was extracted from all isolates using a genomic DNA extraction kit. Then multiplex polymerase chain reaction (M-PCR) method was performed using specific primers. It was found that 60 K. pneumonia isolates out of 220 samples (27.27%) were confirmed by standard phenotypic tests. The PCR test indicated that 6 (10%) strains carried clbN and clbB genes. The pks positive K. pneumoniae was more prevalent in our samples. Owing to its pleiotropic effects, colibactin profoundly influences cellular physiology, inducing DNA breaks that lead to senescence or apoptosis. It seems that the identification of the pks positive K. pneumoniae in milk is essential to prevent colorectal cancer.
کلیدواژه‌ها [English]
)