عنوان مقاله [English]
Annually, many reports of occurrence of food poisoning due to the consumption of sea-foods contaminated with Vibrio species have been reported. The present survey was carried out to evalution the frequency rate of Vibrio species in sea-food products from the fish samples in Isfahan, Iran.
In this survey, 64 fresh fish samples were purchased from markets in Isfahan and transferred to the food quality control laboratory of the Islamic Azad University of Shahreza . Samples were tested for count Vibrio parahaemolyticus and detection according to Iranian national standard methods . positive specimens were evaluated for presence of bacterial species using Nested Polymerase Chain Reaction . the presences of Vibrio parahaemolyticus, Vibrio cholera, Vibrio vulnificus and Vibrio mimicus were studied using the Nested PCR method. Results showed that 95% of samples were contaminated with Vibrio species. In studied samples, V. parahaemolyticus had the highest frequency rate (35%), while V. mimicus had the lowest frequency rate (15%). Frequency of V. cholera and V. vulnificus were 20% and 35% respectively.
The results of microbial investigations of raw fish collected from different markets of Isfahan can not be defined satisfactorily . High prevalence of Vibrio species in samples confirmed the lack of hygienic condition in the production and distribution centers of fish . It seems that, places of fishing and processing and the moods of transportation and distribution of fish don’t have suitable hygiene in Isfahan .
Key Words: PCR, Vibrio, Fish, Contamination